Lab Analysis
At the end of the 20th century, spectacular discoveries high in the Andes—on the mountain peaks in Argentina and Peru—shed new light on Inca sacrificial practices and the daily life of their communities. Thanks to the finding of almost perfectly preserved, naturally mummified bodies of children sacrificed—among others on the summit of Llullaillaco—it became possible to conduct advanced laboratory analyses, which provided many new insights into the religiosity and living conditions within the Inca Empire. Research carried out by an international team of scientists utilized a range of modern methods, from isotope analyses, through genetics, to toxicology and dating.
Stable isotopes of carbon and nitrogen provided information about the diet of the victims in the last months of their lives. Strontium and oxygen isotopes from teeth and hair allowed researchers to determine the origins of the children and their migrations—many of them traveled hundreds of kilometers from various regions of the Inca Empire before reaching the sacrificial site. DNA analyses revealed the genetic diversity of the victims, shedding light on population structure and possible kinship ties, while microbiome studies provided unique data on their health and the environment in which they lived. Toxicological traces of coca leaves and harmine consumption indicate that the children were under the influence of psychoactive substances—likely to alleviate stress. Precise dating of short-lived plants, such as corn seeds and coca leaves found in the graves, enabled accurate dating of the ritual. All these data together create a coherent, multilayered picture of the complex religious rituals of the Incas and allow reconstruction of both the course of the ceremonies and the lives of the children who participated in them.
The latest research results can be followed in Publications.

Toxicological Analysis
Toxicological analyses of hair and nail samples from children sacrificed on the Ampato volcano revealed the presence of harmine and harmaline, likely derived from the Banisteriopsis caapi. Another detected compound was cocaine, resulting from the ritual victims chewing coca leaves. These leaves were used as a remedy for altitude sickness and, additionally, the substances they contain enhance overall physical performance. The Incas highly valued coca leaves and often offered them as sacrifices to gods and the deceased.
The research was conducted in collaboration with the Department of Forensic Medicine at the Nicolaus Copernicus University Medical College in Bydgoszcz.



Ancient DNA analysis
An exceptional case for ancient DNA research is the frozen Inca mummies, particularly the “Children of Llullaillaco.” In collaboration with the Museo de Arqueología de Alta Montaña in Salta, Argentina, and geneticists from the University of Vienna, we conducted non-invasive sampling of the mummies. Thanks to extensive sequencing, we recovered nuclear DNA from the studied individuals. We used this data to determine the kinship of the Llullaillaco children with pre-Columbian and contemporary populations. The non-invasive approach also allowed the detection of commensal and pathogenic microorganism species.

Research on the chronology of the Inca Empire
As part of the project, advanced research is being conducted on the chronology of the development of the Inca State (Tawantinsuyu), with an emphasis on the period of its greatest expansion—from the late 14th century until the arrival of the Spanish in 1532. The goal of the project is to precisely determine the dates of key historical events through the use of radiocarbon and dendrochronological methods.
Badania realizowane są we współpracy z Politechniką Śląską pod kierownictwem dr Dominika Sieczkowskiej-Jacyny i finansowane ze środków Narodowego Centrum Nauki, Polska (2024/52/C/ST10/00061).



Isotopic Research
Isotope studies aim to reconstruct the diet and origin of the victims. Carbon isotope analysis makes it possible to distinguish between plants using the C4 photosynthesis pathway (such as maize) and C3 plants (e.g., potatoes, legumes, cucurbits, or fruits). Analysis of strontium and oxygen isotope ratios helps determine the geological formation and altitude above sea level where the children spent their early years. Similar analyses performed on botanical remains are used to calibrate C14 dating and to reconstruct the climate.
The research is being conducted in collaboration with the AGH University of Kraków and the Max Planck Institute in Jena, Germany.
